{"id":902,"date":"2026-07-01T08:00:00","date_gmt":"2026-07-01T08:00:00","guid":{"rendered":"https:\/\/projekti.imi.hr\/biomoltox\/?p=902"},"modified":"2026-09-18T10:43:21","modified_gmt":"2026-09-18T10:43:21","slug":"fisetin-attenuates-oxidative-and-haemolytic-damage-induced-by-quinuclidine-derivatives-in-human-erythrocytes","status":"publish","type":"post","link":"https:\/\/projekti.imi.hr\/biomoltox\/2026\/07\/01\/fisetin-attenuates-oxidative-and-haemolytic-damage-induced-by-quinuclidine-derivatives-in-human-erythrocytes\/","title":{"rendered":"Fisetin attenuates oxidative and haemolytic damage induced by quinuclidine derivatives in human erythrocytes"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>Autori:&nbsp;<\/strong>Lucija Marceli\u0107, Lea Malezan, Katja Vukovi\u0107, Maja Katalini\u0107, Antonio Zandona<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Sa\u017eetak rada<\/strong><br>Quinuclidine derivatives are potent bioactive compounds investigated as cholinesterase inhibitors with potential intravenous therapeutic application. However, the cytotoxic properties of some, particularly those linked to oxidative stress, pose significant safety concerns. This study aimed to evaluate if flavonoid fisetin could protect cells from such toxic effects. Erythrocytes were chosen due to high susceptibility to reactive oxygen species (ROS) and well-characterised endogenous antioxidant system, making them a sensitive and physiologically relevant model. Over 24 h, human erythrocytes were exposed to QOH-C12\u201316 or QNOH-C12\u201316 derivatives to establish their 25, 50, and 75 % inhibitory concentrations (IC<sub>25<\/sub>, IC<sub>50<\/sub>, and IC<sub>75<\/sub>, respectively). As the compounds with the C12 side alkyl chain showed no significant haemolytic effects, they were excluded from further experimentation. In the next steps, we assessed the protective effects of fisetin (1, 10, 100 \u03bcmol\/L) against quinuclidine-induced haemolysis over 24 h and against reactive oxygen species (ROS) generation and potential glutathione (GSH) and superoxide dismutase (SOD) depletion over 4 h. Fisetin at 10 and 100 \u03bcmol\/L significantly reduced ROS to near-control levels, restored SOD activity, and decreased haemolysis by 10\u201340 %, most effectively in cells exposed to quinuclidines at IC<sub>50<\/sub>. However, GSH measurements in combined treatment were not reliable, as fisetin exhibited autofluorescence, but single quinuclidine exposure showed no significant effects on GSH levels. In summary, fisetin showed a promising potential to make quinuclidine intravenous therapies safer.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Rad je dostupan na sljede\u0107oj&nbsp;<a href=\"https:\/\/arhiv.imi.hr\/index.php\/arhiv\/article\/view\/2010\" data-type=\"link\" data-id=\"https:\/\/arhiv.imi.hr\/index.php\/arhiv\/article\/view\/2010\" target=\"_blank\" rel=\"noopener\">poveznici<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Autori:&nbsp;Lucija Marceli\u0107, Lea Malezan, Katja Vukovi\u0107, Maja Katalini\u0107, Antonio Zandona Sa\u017eetak radaQuinuclidine derivatives are potent bioactive compounds investigated as cholinesterase inhibitors with potential intravenous therapeutic application. However, the cytotoxic properties of some, particularly those linked to oxidative stress, pose significant safety concerns. This study aimed to evaluate if flavonoid fisetin could protect cells from such &hellip;<br \/><a href=\"https:\/\/projekti.imi.hr\/biomoltox\/2026\/07\/01\/fisetin-attenuates-oxidative-and-haemolytic-damage-induced-by-quinuclidine-derivatives-in-human-erythrocytes\/\" class=\"more-link pen_button pen_element_default pen_icon_arrow_double\">Continue reading <span class=\"screen-reader-text\">Fisetin attenuates oxidative and haemolytic damage induced by quinuclidine derivatives in human erythrocytes<\/span><\/a><\/p>\n","protected":false},"author":12,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[13],"tags":[],"class_list":["post-902","post","type-post","status-publish","format-standard","hentry","category-pub-hr"],"acf":[],"_links":{"self":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/902","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/comments?post=902"}],"version-history":[{"count":3,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/902\/revisions"}],"predecessor-version":[{"id":907,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/902\/revisions\/907"}],"wp:attachment":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/media?parent=902"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/categories?post=902"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/tags?post=902"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}