{"id":855,"date":"2026-01-10T08:00:00","date_gmt":"2026-01-10T08:00:00","guid":{"rendered":"https:\/\/projekti.imi.hr\/biomoltox\/?p=855"},"modified":"2026-01-16T08:58:31","modified_gmt":"2026-01-16T08:58:31","slug":"sex-related-expression-of-klotho-in-rat-kidneys-species-differences-between-rats-and-mice-2","status":"publish","type":"post","link":"https:\/\/projekti.imi.hr\/biomoltox\/en\/2026\/01\/10\/sex-related-expression-of-klotho-in-rat-kidneys-species-differences-between-rats-and-mice-2\/","title":{"rendered":"Sex-Related Expression of Klotho in Rat Kidneys: Species Differences Between Rats and Mice"},"content":{"rendered":"\n<p><strong>Authors:\u00a0<\/strong>Davorka Breljak, Dean Karaica, Ivana Vrhovac Maduni\u0107, Vedran Micek, Tatjana Orct, Marija Ljubojevi\u0107, Dubravka Ra\u0161i\u0107, \u017deljka Vogrinc, Sa\u0161a Kralik, Marko Geri\u0107, Goran Gajski, Ivana Novak Jovanovi\u0107, Lucia Nani\u0107, Jasna Jurasovi\u0107, Maja Peraica, Ivica Rubelj, Ivan Saboli\u0107<\/p>\n\n\n\n<p><strong>Abstract<\/strong><br>The anti-aging gene\/protein Klotho (Kl), most present in kidneys, has been well studied in mice (mKl), but not in rats (rKl). This study investigated the renal rKl expression in male and female rats. Sex-related measurement of rKl-controlled electrolytes was performed in plasma\/urine samples, as were tests on species differences in renal Kl expression (rats vs. mice). rKl mRNA\/protein expression was studied by qRT-PCR\/Western-blotting in renal total RNA\/cell membranes and its localization by immunofluorescence microscopy. Urine\/plasma ions (phosphate\/total calcium) and macroelements (phosphorus\/calcium) were measured biochemically and by ICP-MS, respectively. In rat kidneys, the rKl mRNA\/protein was detected in the cortex, outer and inner stripe but not in the papilla, and was immunolocalized in the basolateral membrane of proximal tubules in the cortex and outer stripe, but not in the intercalating cells of the cortical distal tubules, whereas mKl was observed in the mouse kidney cortex but not the outer stripe. Female-dominant expression of renal rKl, affected by androgen\u2019s inhibitory effect, may have contributed to the sex-related level of urine electrolytes, particularly phosphates. Renal mKl expression was male-dominant. Sex- and species-related differences in renal Kl expression may be relevant for the selection of the sex and\/or the model organism in studies addressing aging\/mineral homeostasis.<\/p>\n\n\n\n<p>The article is available at the following\u00a0<a href=\"https:\/\/www.mdpi.com\/1422-0067\/27\/2\/716\" target=\"_blank\" rel=\"noreferrer noopener\">link<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Authors:\u00a0Davorka Breljak, Dean Karaica, Ivana Vrhovac Maduni\u0107, Vedran Micek, Tatjana Orct, Marija Ljubojevi\u0107, Dubravka Ra\u0161i\u0107, \u017deljka Vogrinc, Sa\u0161a Kralik, Marko Geri\u0107, Goran Gajski, Ivana Novak Jovanovi\u0107, Lucia Nani\u0107, Jasna Jurasovi\u0107, Maja Peraica, Ivica Rubelj, Ivan Saboli\u0107 AbstractThe anti-aging gene\/protein Klotho (Kl), most present in kidneys, has been well studied in mice (mKl), but not in &hellip;<br \/><a href=\"https:\/\/projekti.imi.hr\/biomoltox\/en\/2026\/01\/10\/sex-related-expression-of-klotho-in-rat-kidneys-species-differences-between-rats-and-mice-2\/\" class=\"more-link pen_button pen_element_default pen_icon_arrow_double\">Continue reading <span class=\"screen-reader-text\">Sex-Related Expression of Klotho in Rat Kidneys: Species Differences Between Rats and Mice<\/span><\/a><\/p>\n","protected":false},"author":12,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[21],"tags":[],"class_list":["post-855","post","type-post","status-publish","format-standard","hentry","category-pub-en"],"acf":[],"_links":{"self":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/855","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/comments?post=855"}],"version-history":[{"count":1,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/855\/revisions"}],"predecessor-version":[{"id":856,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/855\/revisions\/856"}],"wp:attachment":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/media?parent=855"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/categories?post=855"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/tags?post=855"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}