{"id":783,"date":"2025-09-04T08:00:00","date_gmt":"2025-09-04T08:00:00","guid":{"rendered":"https:\/\/projekti.imi.hr\/biomoltox\/?p=783"},"modified":"2025-09-12T07:58:36","modified_gmt":"2025-09-12T07:58:36","slug":"simplified-assessment-of-acetylcholinesterase-inhibition-by-environmental-organophosphorus-pesticides","status":"publish","type":"post","link":"https:\/\/projekti.imi.hr\/biomoltox\/2025\/09\/04\/simplified-assessment-of-acetylcholinesterase-inhibition-by-environmental-organophosphorus-pesticides\/","title":{"rendered":"Simplified assessment of acetylcholinesterase inhibition by environmental organophosphorus pesticides"},"content":{"rendered":"\n<p><strong>Autori:\u00a0<\/strong>Ruihong\u00a0Zhu,\u00a0Yiyun\u00a0Liu,\u00a0Yongchao\u00a0Ma,\u00a0Tong\u00a0Xu,\u00a0Guanglei\u00a0Yang,\u00a0Antonio\u00a0Zandona,\u00a0Tena\u00a0\u010cade\u017e,\u00a0Zrinka\u00a0Kovarik,\u00a0Heidi Qunhui\u00a0Xie,\u00a0Li\u00a0Xu,\u00a0Bin\u00a0Zhao<\/p>\n\n\n\n<p><strong>Sa\u017eetak rada<\/strong><br>Environmental contaminants, such as pesticides, can inhibit the enzymatic activity of acetylcholinesterase (AChE), an enzyme necessary for neurotransmission. The inhibitory effects of structurally diverse pesticides on AChE may result from either reversible or covalent interactions. Therefore, assessing their potency typically requires different assay design to determine either dissociation constants or rate constants, respectively. To avoid complex kinetic experiments and enable comparison of potencies across structurally diverse pesticides, we optimized an AChE inhibitor detection system using an endpoint Ellman assay in 96-well plates. Given the significant interspecies variability in sensitivity to inhibitors, we investigated AChE inhibition using both electric eel AChE (eeAChE) and human AChE (hAChE). After confirming the repeatability, reproducibility, and solvent compatibility of the detection system using the reversible inhibitor BW284c51, we determined the inhibition potency of selected organophosphorus (OP) pesticides based on IC<sub>50<\/sub>\u00a0values. We found that chlorpyrifos, fenamiphos and ethoprophos were more potent inhibitors of hAChE than eeAChE. In contrast, phosalone and methamidophos showed similar inhibitory effects on both enzymes. The potencies aligned well with previously reported inhibitory rate constants. In conclusion, since OPs acts as progressive inhibitors of AChE, our optimized assay offers a simplified yet effective method for assessing their inhibitory potency. It also allows for comparative evaluation of various environmental pollutants based solely on IC<sub>50<\/sub>\u00a0values, eliminating the need for complex kinetic studies.<\/p>\n\n\n\n<p>Rad je dostupan na sljede\u0107oj\u00a0<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0009279725003527\" data-type=\"link\" data-id=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0009279725003527\" target=\"_blank\" rel=\"noreferrer noopener\">poveznici<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Autori:\u00a0Ruihong\u00a0Zhu,\u00a0Yiyun\u00a0Liu,\u00a0Yongchao\u00a0Ma,\u00a0Tong\u00a0Xu,\u00a0Guanglei\u00a0Yang,\u00a0Antonio\u00a0Zandona,\u00a0Tena\u00a0\u010cade\u017e,\u00a0Zrinka\u00a0Kovarik,\u00a0Heidi Qunhui\u00a0Xie,\u00a0Li\u00a0Xu,\u00a0Bin\u00a0Zhao Sa\u017eetak radaEnvironmental contaminants, such as pesticides, can inhibit the enzymatic activity of acetylcholinesterase (AChE), an enzyme necessary for neurotransmission. The inhibitory effects of structurally diverse pesticides on AChE may result from either reversible or covalent interactions. Therefore, assessing their potency typically requires different assay design to determine either dissociation constants or rate constants, &hellip;<br \/><a href=\"https:\/\/projekti.imi.hr\/biomoltox\/2025\/09\/04\/simplified-assessment-of-acetylcholinesterase-inhibition-by-environmental-organophosphorus-pesticides\/\" class=\"more-link pen_button pen_element_default pen_icon_arrow_double\">Continue reading <span class=\"screen-reader-text\">Simplified assessment of acetylcholinesterase inhibition by environmental organophosphorus pesticides<\/span><\/a><\/p>\n","protected":false},"author":12,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[13],"tags":[],"class_list":["post-783","post","type-post","status-publish","format-standard","hentry","category-pub-hr"],"acf":[],"_links":{"self":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/783","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/comments?post=783"}],"version-history":[{"count":1,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/783\/revisions"}],"predecessor-version":[{"id":784,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/783\/revisions\/784"}],"wp:attachment":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/media?parent=783"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/categories?post=783"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/tags?post=783"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}