{"id":222,"date":"2024-03-10T08:00:00","date_gmt":"2024-03-10T08:00:00","guid":{"rendered":"https:\/\/projekti.imi.hr\/biomoltox\/?p=222"},"modified":"2025-09-29T13:00:51","modified_gmt":"2025-09-29T13:00:51","slug":"use-of-natural-deep-eutectic-solvent-nades-as-a-green-extraction-of-antioxidant-polyphenols-from-strawberry-tree-fruit-arbutus-unedo-l-an-optimization-study","status":"publish","type":"post","link":"https:\/\/projekti.imi.hr\/biomoltox\/2024\/03\/10\/use-of-natural-deep-eutectic-solvent-nades-as-a-green-extraction-of-antioxidant-polyphenols-from-strawberry-tree-fruit-arbutus-unedo-l-an-optimization-study\/","title":{"rendered":"Use of natural deep eutectic solvent (NADES) as a green extraction of antioxidant polyphenols from strawberry tree fruit (Arbutus unedo\u00a0L.): An optimization study"},"content":{"rendered":"\n<p><strong>Autori:&nbsp;<\/strong>Sanja Milo\u0161evi\u0107, Anica Bebek Markovinovi\u0107, Nemanja Tesli\u0107, Aleksandra Mi\u0161an, Milica Poji\u0107, Irena Br\u010di\u0107 Kara\u010donji, Karlo Jurica, Dario Lasi\u0107, Predrag Putnik, Danijela Bursa\u0107 Kova\u010devi\u0107, Branimir Pavli\u0107<\/p>\n\n\n\n<p><strong>Sa\u017eetak rada<br><\/strong>Strawberry tree fruits (<em>Arbutus unedo<\/em>&nbsp;L.) are being studied as an underutilized wild fruit, rich in various bioactive compounds, especially polyphenols. The main objective of this study was to optimize the extraction process using a natural deep eutectic solvent (NADES) to obtain the highest polyphenol content and antioxidant activity of strawberry tree fruits as determined by different&nbsp;<em>in vitro<\/em>&nbsp;assays (DPPH, FRAP and ABTS). For this purpose, extraction with NADES was performed in three steps. Among twenty different NADES systems screened for extraction in the first step of solvent screening, two solvents that gave the best results (N6: Betaine\u2013glycerine\u2013water with a molar ratio 1:2:1 and N9: Choline chloride\u2013glycerine with a molar ratio 1:2) were screened in the next step. For these two solvents, two separate Face-centered central composite designs with S\/L ratios (10\u201320-30&nbsp;g NADES\/g sample), temperature (40\u201355-70&nbsp;\u00b0C), and extraction time (60\u2013120-180&nbsp;min) were used by response surface methodology (RSM), with total phenolic content and antioxidant activity as the target responses. Based on the software multi-response optimization prediction, the optimal conditions for N6 and N9 were: 1:30 S\/L ratio, 65&nbsp;\u00b0C temperature and an extraction time of 150&nbsp;min, and 1:20 S\/L ratio, 56&nbsp;\u00b0C and 180&nbsp;min, respectively. The optimal parameters were verified experimentally and the main polyphenols were quantified by HPLC-DAD.<\/p>\n\n\n\n<p>Rad je dostupan na sljede\u0107oj <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0026265X24003965\" target=\"_blank\" rel=\"noreferrer noopener\">poveznici<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Autori:&nbsp;Sanja Milo\u0161evi\u0107, Anica Bebek Markovinovi\u0107, Nemanja Tesli\u0107, Aleksandra Mi\u0161an, Milica Poji\u0107, Irena Br\u010di\u0107 Kara\u010donji, Karlo Jurica, Dario Lasi\u0107, Predrag Putnik, Danijela Bursa\u0107 Kova\u010devi\u0107, Branimir Pavli\u0107 Sa\u017eetak radaStrawberry tree fruits (Arbutus unedo&nbsp;L.) are being studied as an underutilized wild fruit, rich in various bioactive compounds, especially polyphenols. The main objective of this study was to optimize &hellip;<br \/><a href=\"https:\/\/projekti.imi.hr\/biomoltox\/2024\/03\/10\/use-of-natural-deep-eutectic-solvent-nades-as-a-green-extraction-of-antioxidant-polyphenols-from-strawberry-tree-fruit-arbutus-unedo-l-an-optimization-study\/\" class=\"more-link pen_button pen_element_default pen_icon_arrow_double\">Continue reading <span class=\"screen-reader-text\">Use of natural deep eutectic solvent (NADES) as a green extraction of antioxidant polyphenols from strawberry tree fruit (Arbutus unedo\u00a0L.): An optimization study<\/span><\/a><\/p>\n","protected":false},"author":12,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[13],"tags":[],"class_list":["post-222","post","type-post","status-publish","format-standard","hentry","category-pub-hr"],"acf":[],"_links":{"self":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/222","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/comments?post=222"}],"version-history":[{"count":3,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/222\/revisions"}],"predecessor-version":[{"id":562,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/posts\/222\/revisions\/562"}],"wp:attachment":[{"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/media?parent=222"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/categories?post=222"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/projekti.imi.hr\/biomoltox\/wp-json\/wp\/v2\/tags?post=222"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}