Author:jmadunic

Authors: Mirela Pavić Vulinović, Vedran Micek, Davorka Breljak, Ivana Vrhovac Madunić, Josip Madunić, Marija Ljubojević AbstractZinc is an essential micronutrient involved in structural, catalytic, and regulatory functions across all levels of biological organization. Despite substantial advances over the past two decades, the zinc literature remains highly fragmented, with mechanistic, nutritional, and clinical findings often reported …
Continue reading Overview of the Zinc Functional Interactome Through Health Hallmarks and Medical Conditions

Authors: Davorka Breljak, Dean Karaica, Ivana Vrhovac Madunić, Vedran Micek, Tatjana Orct, Marija Ljubojević, Dubravka Rašić, Željka Vogrinc, Saša Kralik, Marko Gerić, Goran Gajski, Ivana Novak Jovanović, Lucia Nanić, Jasna Jurasović, Maja Peraica, Ivica Rubelj, Ivan Sabolić AbstractThe anti-aging gene/protein Klotho (Kl), most present in kidneys, has been well studied in mice (mKl), but not in …
Continue reading Sex-Related Expression of Klotho in Rat Kidneys: Species Differences Between Rats and Mice

Authors: Martin Češi, Andreja Jurič, Marko Obranović, Danijela Šeremet, Manuela Panić, Nevenka Kopjar, Maja Katalinić, Snježana Džijan, Antonio Zandona AbstractThe development of a new solvent useful in extractions of bioactive compounds from waste plant material presents a great challenge for researchers. Nowadays, deep eutectic solvents (DES) have become attractive since they can be formed by mixing different biologically non-harmful constituents. Although its components are considered safe, any newly formulated solvent must still undergo …
Continue reading New insights into fatty acid-based deep eutectic solvent as a carrier forcannabidiol: Cytotoxic and genotoxic effects in human astrocytoma cells

Authors: Nikolina Maček Hrvat, Borna Puljko, Rakesh K Sit, Katarina Ilić, Dora Kolić, Kristina Mlinac-Jerkovic, Svjetlana Kalanj-Bognar, Zoran Radić, Barry K Sharpless, Palmer Taylor, Zrinka Kovarik AbstractToxic organophosphates like the nerve agent sarin readily cross the blood-brain barrier (BBB) and inhibit acetylcholinesterase (AChE), a pivotal enzyme in regulating neurotransmission by hydrolysis of acetylcholine (ACh). Elevated levels …
Continue reading The Ionizing Zwitterionic Oxime Antidote Attenuates Gliosis in Mice Exposed to Sarin

Authors: Zrinka Kovarik, Dora Kolić, Tena Čadež, Goran Šinko, Petra Tuksar, Tamara Zorbaz, Christophe Curty, Nikolina Maček Hrvat AbstractGiven the scarcity of experimental studies on A-series nerve agents (NAs), this paper provides ground-breaking insights and, for the first time, describes the inhibition and reactivation of human acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibited by these NAs using standard oximes. Furthermore, we present a detailed assessment of the toxicity profile of A-series NAs, …
Continue reading Outlining the A-series of organophosphorus compounds: Cholinesterase inhibition, reactivation, cytotoxicity, and acute toxicity in mice

Authors: Antonio Zandona, Lucija Marcelić, Suzana Žunec, Josip Madunić, Maja Katalinić AbstractHaving a broad spectrum of biological and pharmacological activities, including anticholinergic, antihistaminic, antiparasitic, antioxidant, and antitumour, quinuclidine derivatives have piqued much interest in the field of drug discovery and biomedical research. This study investigates the oxidative stress effects of six N‐alkyl quaternary quinuclidine derivatives, namely three …
Continue reading Effects of N‐alkyl quaternary quinuclidines on oxidative stress biomarkers in SH-SY5Y cells

Authors: Ruihong Zhu, Yiyun Liu, Yongchao Ma, Tong Xu, Guanglei Yang, Antonio Zandona, Tena Čadež, Zrinka Kovarik, Heidi Qunhui Xie, Li Xu, Bin Zhao AbstractEnvironmental contaminants, such as pesticides, can inhibit the enzymatic activity of acetylcholinesterase (AChE), an enzyme necessary for neurotransmission. The inhibitory effects of structurally diverse pesticides on AChE may result from either reversible or covalent interactions. Therefore, assessing their potency typically requires different assay design to determine either dissociation constants or rate constants, respectively. …
Continue reading Simplified assessment of acetylcholinesterase inhibition by environmental organophosphorus pesticides