Autori: Martin Češi, Andreja Jurič, Marko Obranović, Danijela Šeremet, Manuela Panić, Nevenka Kopjar, Maja Katalinić, Snježana Džijan, Antonio Zandona
Sažetak rada
The development of a new solvent useful in extractions of bioactive compounds from waste plant material presents a great challenge for researchers. Nowadays, deep eutectic solvents (DES) have become attractive since they can be formed by mixing different biologically non-harmful constituents. Although its components are considered safe, any newly formulated solvent must still undergo thorough toxicological evaluation. In this in vitro study, we focused on basic in vitro toxicity assays on human astrocytoma cell line 1321 N1 to establish cytotoxic and DNA damaging potency of a DES, formed by mixing lauric and octanoic acids. The observed effects were compared with ethanol, which is used as a standard solvent for extraction of many bioactive components. The experimental model involved 24 h exposure of 1321 N1 cells to three chosen volumes of DES or ethanol, added in the culture medium. The same endpoints were evaluated in cells exposed to the cannabidiol (CBD) extract in DES and in the ethanolic solution prepared at equivalent CBD concentrations. Obtained results reveal that 24 h exposure to DES and its CBD extract produced minor cytotoxic effects, without significant impairments of DNA integrity in 1321 N1 cells. Both cytotoxic and DNA damaging effects were pronounced more in the ethanolic samples. Although promising, these observations should be considered preliminary, due to the limitations of the applied test system. Further investigations using a wide range of tests will be performed to better clarify the safety and toxicological profile of this solvent.
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