Exploring cytokinesis block micronucleus assay in Croatia: A journey through the past, present, and future in biomonitoring of the general population

Posted on Updated on by jmadunicKategorije:Publikacije

Autori: Goran Gajski, Vilena Kašuba, Mirta Milić, Marko Gerić, Katarina Matković, Luka Delić, Maja Nikolić, Martina Pavičić, Ružica Rozgaj, Vera Garaj-Vrhovac, Nevenka Kopjar

Sažetak rada
In this study, we used the cytokinesis-block micronucleus (CBMN) assay to evaluate the background frequency of cytogenetic damage in peripheral blood lymphocytes of the general population concerning different anthropometric data and lifestyle factors. The background frequency of CBMN assay parameters was analysed in 850 healthy, occupationally non-exposed male and female subjects (average age, 38±11 years) gathered from the general Croatian population from 2000 to 2023. The mean background values for micronuclei (MNi) in the whole population were 5.3±4.3 per 1000 binucleated cells, while the mean frequency of nucleoplasmic bridges (NPBs) was 0.7±1.3 and of nuclear buds (NBUDs) 3.1±3.2. The cut-off value, which corresponds to the 95th percentile of the distribution of 850 individual values, was 14 MNi, 3 NPBs, and 9 NBUDs. Results from our database also showed an association of the tested genomic instability parameters with age and sex but also with other lifestyle factors. These findings underscore the importance of considering several anthropometric and lifestyle factors when conducting biomonitoring studies. Overall, the normal and cut-off values attained here present normal values for the general population that can later serve as baseline values for further human biomonitoring studies either in Croatia or worldwide.

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  1336. Ovaj članak pruža uvid u korištenje testa mikronukleusa s blokadom citokineze u praćenju citogenetskih oštećenja u općoj populaciji. Pristup studije ispitivanju pozadinske učestalosti mikronukleusa u limfocitima periferne krvi u različitim demografskim skupinama ključan je za razumijevanje utjecaja okoliša i načina života na genetsku stabilnost. Detaljno istraživanje ovih testova otvara put učinkovitijim strategijama javnog zdravstva u genetskom praćenju. Fascinantno je vidjeti kako ove metode unapređuju istraživanja biomonitoringa!

  1337. This study explores the use of the cytokinesis-block micronucleus (CBMN) assay to measure genetic damage in the general Croatian population over time. It examines the frequency of micronuclei and other cytogenetic markers in peripheral blood lymphocytes, considering various lifestyle factors. The research found consistent results across 850 healthy individuals, providing insights into cytogenetic damage. The findings highlight the importance of CBMN assays in biomonitoring public health and understanding environmental and genetic influences on the population.

  1365. This study looks at how genetic damage in blood cells is measured in Croatia using a test called the cytokinesis-block micronucleus (CBMN) assay. Researchers checked the number of certain cell abnormalities, like micronuclei, in a group of 850 healthy people over 20 years. They wanted to see how factors like lifestyle and personal habits affect these cell changes. The results help to understand how common this genetic damage is in the general population and could guide future health monitoring. It’s interesting how this kind of study can help improve public health practices.

  1370. This article provides an insightful overview of the application of the cytokinesis-block micronucleus (CBMN) assay in Croatia, highlighting its evolution and significance in biomonitoring. The study’s focus on assessing cytogenetic damage in peripheral blood lymphocytes offers valuable data on the impact of various factors such as age, sex, seasonal variations, and lifestyle on genomic stability within the general population.

  1392. U ovom istraživanju korištena je metoda blokade citokineze za procjenu učestalosti citogenetskih oštećenja u perifernim limfocitima krvi opće populacije u Hrvatskoj. Analizirani su podaci od 850 zdravih osoba različite dobi i spola, prikupljeni od 2000. do 2023. godine. Rezultati pokazuju vrijednosti mikronukleusa i drugih pokazatelja genomske nestabilnosti te njihovu povezanost s dobi, spolom i stilom života. Ovi nalazi pomažu u razumijevanju utjecaja okolišnih i životnih čimbenika na genetsko zdravlje populacije.

  1393. This article “Exploring Cytokinesis-Block Micronucleus Assay in Croatia” was quite interesting and informative. I understood how genomic instability and cytogenetic damage are measured in the general population through the CBMN assay. The impact of age, sex, and lifestyle factors was also clearly explained, which is very helpful for the real-life context. The discussion of data and cut-off values is valuable for future research. If the article had some simple diagrams or charts, it would have been easier to understand complex numbers. Overall, it is quite useful and educational content, I would definitely recommend it.

  1397. In this study, the cytokinesis-block micronucleus (CBMN) assay was used to evaluate the frequency of cytogenetic damage in peripheral blood lymphocytes from the general Croatian population, analyzing 850 healthy, non-exposed individuals over the years 2000 to 2023. The study measured micronuclei (MN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs), which are key indicators of genomic instability. The results highlight the importance of considering various factors like age, sex, and lifestyle when performing biomonitoring studies. These findings provide baseline values for future studies on human biomonitoring, both in Croatia and worldwide.

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  1432. I just read the insightful article on the cytokinesis-block micronucleus assay in Croatia. It’s heartening to see how this research is advancing our understanding of genetic health in the general population. Kudos to the team for their dedication and meticulous work. Wishing you all continued success in your groundbreaking endeavors! 🌱🔬

  1434. This is a highly insightful article on the CBMN assay and its application in biomonitoring the general population. I appreciate the detailed analysis of cytogenetic damage in relation to age, sex, and lifestyle factors. The comprehensive data from 850 healthy participants provide a valuable baseline for future studies in Croatia and beyond. It’s impressive how the authors connect scientific rigor with practical relevance, making it a useful resource for researchers and public health professionals alike.

  1435. This is a really interesting study! I didn’t know much about the CBMN assay before, but now I understand how important it is for checking cell damage and health in people. It’s great that you used data from so many years and from a wide range of people. I also like that you looked at how age, sex, and lifestyle can affect the results. This kind of research is very helpful for future health studies in Croatia and other countries too. Thank you to the whole team for sharing this important work with the public!

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